FastQCFastQC Report
Thu 2 Jun 2016
DRR028464_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028464_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1653949
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26230.15859013790630788No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC18450.049.8789024
CAACGCA18500.049.744095
ATCAACG20100.046.505363
GTATCAA22100.043.9633181
AACGCAG21550.041.6710936
GGTATCA15350.039.320071
CGCAGAG23100.036.7208568
ACGCAGA23950.035.6512537
GTACATG36100.035.0695421
TATCAAC28450.032.857182
TACATGG37600.032.4959342
ACATGGG42200.027.557513
GCAGAGT29900.025.7023949
GAGTACA26300.020.9900911
CATGGGG29000.020.5631794
GTATTAG14400.020.4459041
GTATATA19100.018.8830221
GTATAAG11600.018.4013161
CATGGGA21600.017.7236354
TGGACCG2650.001857796116.6689625
Produced by FastQC (version 0.11.5)