FastQCFastQC Report
Thu 2 Jun 2016
DRR028464_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028464_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1653949
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19900.066.286041
GTATCAA35150.061.446161
TCAACGC36550.056.072284
ATCAACG37550.054.7718663
CAACGCA38300.053.888395
AACGCAG41850.049.317216
CGCAGAG44250.045.169468
TATCAAC46350.044.685382
ACGCAGA46950.043.343097
GCAGAGT55250.034.7346349
GTACATG36100.034.532261
TACATGG40400.029.9353432
ACATGGG39750.028.9673733
CATGGGG28250.023.8404054
CCGGTCG1851.8453968E-423.487069
CAGAGTA55200.020.3348879
GAGTACA27800.019.2925971
TATACTG14750.018.1659815
TAATACA21950.017.8159244
CCTATAC7301.8189894E-1216.8645673
Produced by FastQC (version 0.11.5)