FastQCFastQC Report
Thu 2 Jun 2016
DRR028463_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028463_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1713665
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26880.15685679523127333No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC20400.048.001734
CAACGCA21350.046.5555345
GTATCAA24050.045.001261
ATCAACG21550.044.7488023
GGTATCA16750.041.3176351
AACGCAG24600.040.3964356
GTACATG38500.038.8201941
CGCAGAG25800.034.585288
TACATGG38550.034.1821252
TATCAAC31500.032.9517862
ACGCAGA28400.032.6595047
ACATGGG41150.030.9480763
GCAGAGT32400.023.7351729
GAGTACA26450.021.7115761
CATGGGG29100.021.5060844
CATGGGA22750.021.0362034
TGAACCG2658.309775E-519.4485655
GTATTAG13050.018.6176931
GAACCGT2851.4542497E-418.0799646
GTATAGA13500.016.906371
Produced by FastQC (version 0.11.5)