FastQCFastQC Report
Thu 2 Jun 2016
DRR028463_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028463_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1713665
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20200.061.70941
GTATCAA35650.054.074981
TCAACGC37900.049.1064534
ATCAACG38750.048.403053
CAACGCA40250.046.059455
AACGCAG43950.042.5114066
TATCAAC47900.039.4593282
CGCAGAG47500.038.5719458
ACGCAGA49550.037.4145937
GTACATG41550.032.617061
TACATGG41850.030.6282712
GCAGAGT57350.030.5581199
ACATGGG41350.029.5975553
CATGGGG26150.026.0315994
CAGAGTA56550.023.8190829
TATACCG1700.00311413421.299325
CGTGTCC2404.403601E-521.1218268
ATACCGT2506.0301623E-520.2769556
TCTATAC13200.019.7502783
GTATTAG13900.019.2912861
Produced by FastQC (version 0.11.5)