FastQCFastQC Report
Thu 2 Jun 2016
DRR028462_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028462_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10453
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.42093178991677027No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA310.2965655792595427No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA240.22959915813642018No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA210.20089926336936764No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN160.1530661054242801No Hit
CAGTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTA130.1243662106572276No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA130.1243662106572276No Hit
GCATTGCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATC110.10523294747919257No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACAGA203.5264436E-4110.48026
ATCAACA258.5638143E-488.384162
GGTATCA258.5638143E-488.384161
GAGAACA300.001766374873.6534651
GTATCAA300.001766374873.6534652
CGCAGAG300.001910380172.22339
CAACACA350.00325507563.1315464
TATCAAC701.8916217E-763.1315461
ACGCAGA606.48656E-660.186088
CACAAAA450.00880080549.102317
TCAACGC656.472262E-445.325215
ATCAACG656.472262E-445.325214
AACGCAG750.001309218739.281857
CAACGCA900.00320439432.7348756
Produced by FastQC (version 0.11.5)