DRR028462_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	DRR028462_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10453
Sequences flagged as poor quality	0
Sequence length	151
%GC	43

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	77	0.7366306323543481	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	70	0.6696642112312255	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT	54	0.5165981058069454	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45	0.4304984215057878	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	26	0.2487324213144552	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	25	0.23916578972543767	No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA	22	0.21046589495838514	No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT	21	0.20089926336936764	TruSeq Adapter, Index 1 (95% over 21bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20	0.19133263178035012	No Hit
GTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGG	17	0.1626327370132976	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	17	0.1626327370132976	No Hit
CAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGT	16	0.1530661054242801	No Hit
GCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCAC	16	0.1530661054242801	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	16	0.1530661054242801	No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAG	14	0.1339328422462451	No Hit
ACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGGTGATGCGATGA	13	0.1243662106572276	No Hit
GATTAAAATAATTTGAAGGCGATCTTTTAAAAAGAGATTAAACCGAAGGT	13	0.1243662106572276	No Hit
GGTAAATCATCACGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGT	13	0.1243662106572276	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	13	0.1243662106572276	No Hit
GACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGGTGATG	12	0.11479957906821009	No Hit
GTCTTTGGAATTACATTGTTTATAGTTACTCTAAAACGCTAAGTTAGTTT	12	0.11479957906821009	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	12	0.11479957906821009	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12	0.11479957906821009	No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA	12	0.11479957906821009	No Hit
CCTCCTTTTCAGTAAAACAAGAAGAACCATGGGGGAAAAAAACTCTATAG	12	0.11479957906821009	No Hit
TCTTTGTTCCAATGTAAAGAACACTTATAATCATGAGGAAAGATACACAT	11	0.10523294747919257	No Hit
TCCTAGACCTAACCTGACTAGAAAAGCTATTACCTAAAACAATTTCACAG	11	0.10523294747919257	No Hit
GAATAACACCATAAAAACAACTGCTTTTCAATTTTTCTGTCACTCCTGTC	11	0.10523294747919257	No Hit
GTACATGGGATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCG	11	0.10523294747919257	No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT	11	0.10523294747919257	No Hit
GAATATTTATGTCCTTCAATTCTGATACATTTCTTGAGTTATTTGATTTG	11	0.10523294747919257	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	11	0.10523294747919257	No Hit
ACTCAACTGACCTTTAGGGGGAAAAAATTCAAGTTTACAATAAGGTACTA	11	0.10523294747919257	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGAGAA	10	0.006996997	144.96173	9
ATTACAC	10	0.006996997	144.96173	2
GGTATCA	45	0.0	129.47437	1
CTTACTC	20	3.762682E-4	108.7213	3
GTATCAA	70	2.5465852E-11	82.835266	2
TATCAAC	90	2.382876E-10	64.42744	3
ACTAACA	35	0.0034722015	62.126453	2
AGTACAT	35	0.0034722015	62.126453	7
CTAACAT	35	0.0034722015	62.126453	3
ATCAACG	100	6.0936145E-10	57.984684	4
TCAACGC	105	9.404175E-10	55.223515	5
CAACGCA	105	9.404175E-10	55.223515	6
ACATGGG	40	0.005891491	54.36065	3
CATTTCG	40	0.005891491	54.36065	7
GAGTACA	40	0.005891491	54.36065	6
TAACATT	40	0.005891491	54.36065	4
ATTTCGC	40	0.005891491	54.36065	8
AACGCAG	115	2.1082087E-9	50.42147	7
GTACATG	45	0.0092087155	48.552887	1
GACTAAC	45	0.0092087155	48.552887	1

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content