FastQCFastQC Report
Thu 2 Jun 2016
DRR028461_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028461_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1956079
Sequences flagged as poor quality0
Sequence length151
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30620.1565376449519677No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17950.049.213421
TCAACGC22850.048.6650354
CAACGCA23400.047.2077265
ATCAACG23950.046.105473
GTATCAA29100.040.475641
AACGCAG28400.038.8893626
GTACATG42700.037.2385331
TATCAAC32950.034.4049262
CGCAGAG27600.033.8957068
TACATGG45200.032.7350462
ACGCAGA31850.031.440357
ACATGGG44850.031.1858713
GTATAGG9700.024.2853831
GCAGAGT35850.023.264589
GTATAGA15550.023.1970311
CATGGGA24400.022.3340854
CGACGAC5700.021.467075145
TATACCG2103.8832676E-421.0411575
GAGTACA28150.020.9208011
CATGGGG28350.020.2613224
Produced by FastQC (version 0.11.5)