FastQCFastQC Report
Thu 2 Jun 2016
DRR028461_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028461_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1956079
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21700.060.4364361
GTATCAA36300.049.1030461
TCAACGC36000.047.870254
ATCAACG36800.046.8295943
CAACGCA37050.046.5136035
AACGCAG41550.041.9988376
TATCAAC46800.037.132712
CGCAGAG44400.037.0198028
GTACATG44600.034.4414181
ACGCAGA49100.034.3609927
ACATGGG44550.029.7436733
TACATGG47650.029.3282152
GCAGAGT58700.027.014529
CGTACCC1500.001505781524.1362633
CATGGGG28500.023.3740634
CAGAGTA57100.021.557789
GAGTACA29700.021.4687231
GTATATA19600.021.0716611
TAGTACG3457.6142896E-820.9880544
CCCGTAT2659.398261E-519.139571
Produced by FastQC (version 0.11.5)