FastQCFastQC Report
Thu 2 Jun 2016
DRR028460_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028460_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17235
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.4119524223962866No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA340.19727299100667245No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA320.18566869741804468No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA230.1334493762692196No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN220.12764722947490573No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA210.12184508268059183No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC190.11024078909196403No Hit
GTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGAT190.11024078909196403No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA180.10443864229765012No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATA100.006733631147.100891
CATCGTG100.006733631147.100897
ACATCGT100.006733631147.100896
CCATTAG100.006733631147.100891
ATCGTGA100.007099555144.52778
GGTATCA300.001797210573.5504461
GTATCAA350.003311733263.0432432
GTACATG1053.2651114E-642.0288281
TACATGG906.5901535E-540.861362
ACATGGG1102.147212E-433.4320223
CTGCCGA4353.668897E-45.3005075120-124
Produced by FastQC (version 0.11.5)