FastQCFastQC Report
Thu 2 Jun 2016
DRR028459_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028459_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1060755
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16670.15715221705294813No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC13750.054.5860824
GTATCAA14750.052.3743551
ATCAACG14350.051.775933
CAACGCA14200.050.267685
AACGCAG15500.045.0934376
GGTATCA10000.044.1440961
ACGCAGA16250.041.2031567
TATCAAC19050.040.544482
CGCAGAG16950.037.6528668
TACCGTT1001.2758524E-436.788537
GTACATG24700.036.042081
TACATGG25100.034.288582
CAACGCG1203.7190338E-430.6615565
ACATGGG28650.027.730493
GCAGAGT20050.027.1288879
TAGTACC4800.022.9950544
GAGTACA17450.020.6596091
CGTTATA1850.004640845619.8808842
CATGGGG20600.019.2890574
CATGGGA15400.019.1127744
Produced by FastQC (version 0.11.5)