FastQCFastQC Report
Thu 2 Jun 2016
DRR028459_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028459_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1060755
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14220.13405546049747585No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12780.12048022399140236No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14200.080.6475451
GTATCAA27050.058.949191
TCAACGC30250.051.234524
ATCAACG30350.051.0657043
CAACGCA30700.050.483525
AACGCAG33000.047.184446
TATCAAC35700.043.6158682
ACGCAGA37100.041.3843577
CGCAGAG37250.040.051178
GCAGAGT42550.033.5305679
GTACATG24500.031.3591
CCGCTCG950.00475217930.4937389
TACATGG25300.030.0568222
TATACGG1255.186359E-428.9690515
ACATGGG26850.027.7822363
CAGAGTA41150.026.2235039
TATACTG8100.024.1408755
CATGGGG20350.023.488424
GTGCGCC1851.8440663E-423.4884199
GAGTACA19000.019.8368231
Produced by FastQC (version 0.11.5)