FastQCFastQC Report
Thu 2 Jun 2016
DRR028458_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028458_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences984659
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15000.1523370019468669No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8750.053.852571
ATCAACG12450.049.07673
TCAACGC12800.047.7472044
GTATCAA14950.046.786111
CAACGCA13250.044.4584125
AACGCAG14000.041.0183756
GTACATG24500.039.9687041
TACATGG24550.038.6796152
TATCAAC17950.036.0879482
CTAGCGT1051.6920258E-435.0639274
CGCAGAG15400.033.9082458
ACGCAGA16500.033.022127
ACATGGG28800.031.439763
CATGGGA14650.024.1258954
GAGTACA18450.023.5445381
GCAGAGT20800.022.3168289
ACGATCC3458.098323E-820.863403145
CATGGGG20500.020.8331034
AGTACAT19600.020.6562062
TATTAGG7200.020.447562
Produced by FastQC (version 0.11.5)