FastQCFastQC Report
Thu 2 Jun 2016
DRR028458_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028458_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences984659
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11960.12146336955230186No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10500.1066359013628068No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12200.076.0353241
GTATCAA23500.054.8930551
TCAACGC25900.048.9346774
ATCAACG26250.048.282213
CAACGCA26300.048.1904265
AACGCAG27450.046.1715166
TATCAAC30800.041.6198962
GTACATG26950.039.2608151
ACGCAGA32050.039.0927737
TCTAGCG750.001491108738.625772
TACATGG27900.037.6393622
CGCAGAG32600.037.1002928
ACATGGG28600.035.958433
GTGGTAT6300.034.510081
GCAGAGT38450.028.0652149
CATGGGA15600.026.926624
TGGTATC7900.025.6690272
CAGAGTA38300.022.691389
CATGGGG19900.022.2001134
ACTACGT1650.002616858321.9464633
Produced by FastQC (version 0.11.5)