FastQCFastQC Report
Thu 2 Jun 2016
DRR028457_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028457_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1166056
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18370.1575396035867917No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11900.056.2678261
TCAACGC17000.047.182564
CAACGCA17550.045.286595
GTACATG30450.044.946031
ATCAACG17700.044.4792183
GTATCAA18750.042.775091
AACGCAG19050.040.940916
TACATGG31550.039.4107632
ACGCAGA20400.036.429817
TATCAAC23200.034.567342
CGCAGAG20300.034.284328
ACATGGG35950.032.3373763
CATGGGA16050.024.7584024
GCAGAGT24050.024.7204729
GAGTACA22800.023.558831
ATCGACG1650.002387466622.2962913
GTATCGA1700.002838557821.6414721
CATGGGG25300.021.2327484
GTATAAG7850.020.6214281
AGTACAT24050.019.8850082
Produced by FastQC (version 0.11.5)