FastQCFastQC Report
Thu 2 Jun 2016
DRR028457_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028457_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1166056
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13200.1132021103617665No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12620.1082280782398101No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17000.079.736051
GTATCAA32050.063.3274541
TCAACGC35700.055.987754
ATCAACG35850.055.753493
CAACGCA37050.053.947715
AACGCAG38950.051.687976
TATCAAC40250.049.4787752
ACGCAGA45500.043.928857
CGCAGAG45600.043.67378
GCAGAGT52500.035.8646169
GTACATG30250.031.3912011
ACCGCGT950.00475355430.4921868
TACATGG30450.029.015142
ACATGGG31700.026.5003033
GTGGTAT8550.023.7385371
CATGGGA16400.021.1957874
AGAGTAC45800.019.4487515
CATGGGG20900.019.4041164
GTCTTAC6151.8189894E-1218.858491
CAGAGTA51600.018.5257744
Produced by FastQC (version 0.11.5)