FastQCFastQC Report
Thu 2 Jun 2016
DRR028456_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028456_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3205416
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN51930.1620070530626914No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC36250.053.4739044
ATCAACG38150.050.2158163
CAACGCA39200.048.8872265
AACGCAG40650.046.2264066
GGTATCA28950.043.0130161
GTATCAA49400.040.8681871
GTACATG75600.038.1080471
ACGCAGA48150.037.80297
CGCAGAG46500.036.830078
TACATGG78500.034.2603572
TATCAAC58050.033.636622
ACATGGG74500.033.3300063
CATGGGA47100.025.8200574
GCAGAGT60450.023.6490359
CATGGGG43800.021.3709494
GAGTACA50450.021.0311531
GTATAAT30650.019.9530541
GGCGCGT1850.00502205719.61298
GTATATA30750.017.0127681
CAGAGTA59000.016.9734699
Produced by FastQC (version 0.11.5)