FastQCFastQC Report
Thu 2 Jun 2016
DRR028456_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028456_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3205416
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33700.054.1890141
TCAACGC47600.047.612264
ATCAACG49200.046.3582343
CAACGCA48900.046.198425
AACGCAG53300.042.7922136
GTATCAA56800.042.3575021
GTACATG78750.036.3485531
ACGCAGA63900.035.2404067
CGCAGAG63750.034.755438
TACATGG77700.034.572782
ACATGGG74500.034.211163
TATCAAC71250.032.214832
GCAGAGT79750.025.5127359
CATGGGA46850.025.3463634
CATGGGG38250.021.9587674
GAGTACA54400.019.8485131
GTATTAG27950.019.4455411
CAGAGTA78650.019.0569349
TACCGTA5707.2759576E-1219.054517
TCTATAC25950.018.973743
Produced by FastQC (version 0.11.5)