FastQCFastQC Report
Thu 2 Jun 2016
DRR028455_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028455_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3214417
Sequences flagged as poor quality0
Sequence length151
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN53060.16506881341157667No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC32100.046.174524
ATCAACG33550.044.3873183
CAACGCA34050.043.0977175
GGTATCA28450.039.1311451
AACGCAG39200.037.8040126
GTATCAA45450.035.8497661
GTACATG67050.034.856871
CGCAGAG41250.031.4908268
TACATGG65600.031.466922
ACGCAGA44450.031.3515977
TATCAAC52850.030.1306742
ACATGGG65300.029.5793193
CATGGGG41750.022.9613934
GAGTACA42350.021.7612821
GCAGAGT55300.021.3910059
TCTATAC27750.021.2533363
GTATAGA26050.019.5285171
CAGAGTA53400.018.4827389
GTATAAT34250.017.8667451
CATGGGA40800.017.5316564
Produced by FastQC (version 0.11.5)