FastQCFastQC Report
Thu 2 Jun 2016
DRR028455_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028455_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3214417
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31850.057.5607951
TCAACGC48700.052.186234
ATCAACG51050.049.9257623
CAACGCA50950.049.7395255
GTATCAA60400.044.0296061
AACGCAG57300.043.8482866
CGCAGAG60900.039.591758
TATCAAC68250.037.76812
ACGCAGA65450.037.7244157
GTACATG67850.031.3988111
ACATGGG59000.030.5580163
TACATGG67650.029.0054262
GCAGAGT77000.028.9626169
TCTATAC26800.022.964763
CATGGGG37550.022.1751044
TATACTG32000.021.043155
GAGTACA44900.020.6575741
CAGAGTA76100.019.7904879
CATGGGA34750.019.16954
ATTATAC32600.017.5463713
Produced by FastQC (version 0.11.5)