FastQCFastQC Report
Thu 2 Jun 2016
DRR028454_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028454_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1329772
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21330.16040343758178094No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC15400.056.464844
ATCAACG15500.056.0940323
CAACGCA16650.052.229785
GTATCAA18650.050.570531
GGTATCA11250.049.7768551
AACGCAG18600.046.743226
CGCAGAG20400.040.9194038
TATCAAC22000.040.860492
ACGCAGA20950.039.3897827
GTACATG29200.033.0563471
GCAGAGT24250.029.3343149
TACATGG31000.028.7600772
GTACCGT1657.54456E-526.7927276
ACATGGG33200.025.0787983
TATACCG1600.001980605323.0256
GTACTAT7900.021.4519671
ATACCGC1750.003332429621.0514287
TATAACG1800.003922436420.4674572
GTATATA13700.019.8997461
GAGTACA22600.019.887851
Produced by FastQC (version 0.11.5)