FastQCFastQC Report
Thu 2 Jun 2016
DRR028454_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028454_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1329772
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17260.1297966869508457No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15210.1143805103431265No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17300.076.22591
GTATCAA30450.059.963991
TCAACGC36600.050.0540244
ATCAACG36950.049.57993
CAACGCA38050.048.146585
AACGCAG39900.045.551266
TATCAAC42950.042.9909062
CGCAGAG46000.038.4088758
ACGCAGA46650.038.339367
GCAGAGT52600.032.350559
GTACATG32850.030.6589071
ATACCGC1453.5209203E-529.962837
TACATGG35750.027.3437022
ACATGGG37200.024.5260263
CAGAGTA51050.024.1130859
GTATTAG12250.022.4762971
TATACCG1952.637402E-422.2800526
GTGGTAT10350.021.7019421
TACCGCC2053.6981542E-421.193228
GAGTACA27050.020.08961
Produced by FastQC (version 0.11.5)