FastQCFastQC Report
Thu 2 Jun 2016
DRR028453_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028453_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1424736
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22940.16101228578487525No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC18000.055.7488334
ATCAACG19650.051.0583953
CAACGCA19600.050.8214535
AACGCAG20650.047.5089346
GTATCAA21700.046.5765531
GGTATCA13250.043.4295431
ACGCAGA23950.038.808287
CGCAGAG24000.037.527538
TATCAAC27200.037.4283752
GTACATG33550.035.8427731
TACATGG33900.033.5128862
ACATGGG35600.030.0475333
GCAGAGT27700.028.3203749
CATGGGG22700.025.6785564
CATGGGA18300.020.5630954
TCTGTCG5153.6379788E-1119.7451468
GTATTAG11600.019.715611
GTATTAT14550.019.2675781
GTATATA17050.018.6058961
GTCTACG2851.4295602E-418.1200621
Produced by FastQC (version 0.11.5)