FastQCFastQC Report
Thu 2 Jun 2016
DRR028453_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028453_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1424736
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18400.072.856481
GTATCAA36050.054.8746381
TCAACGC35950.054.1734474
ATCAACG36150.053.8737343
CAACGCA37200.052.3531045
AACGCAG39800.049.114966
CGCAGAG43350.043.756778
ACGCAGA43950.043.65367
TATCAAC45200.043.247242
GCAGAGT50700.034.1289569
GTACATG32850.032.8673861
TACATGG33200.029.6574552
ACATGGG34900.026.34583
CCGTATA1801.5351654E-424.1330269
CAGAGTA49550.021.6247529
GAGTACA24850.021.2868181
CATGGGA17750.020.801994
TAGATCG1750.003689135720.6854535
TCTATAC11700.020.4202563
GTGGTAT10000.020.2895451
Produced by FastQC (version 0.11.5)