FastQCFastQC Report
Thu 2 Jun 2016
DRR028452_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028452_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1742283
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28300.16243055806662868No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC20750.056.136774
ATCAACG21700.052.9808963
CAACGCA21900.051.8439185
AACGCAG22650.050.1153876
GTATCAA24600.048.835161
GGTATCA15250.046.879391
ACGCAGA26150.041.7190327
CGCAGAG25800.040.2453848
GTACATG39550.038.947531
TATCAAC30050.038.7495842
TACATGG43300.035.0617642
ACATGGG48750.029.6302913
CAACGCG1000.0055976129.4904375
GCAGAGT35050.026.9319449
CATGGGG35050.022.50634
GTACGGA2703.8539383E-621.8396056
AGTACGG3207.216713E-720.7354625
CATGGGA22950.020.5591754
TAGTACG4701.891749E-1020.391724
GAGTACA30150.019.5561241
Produced by FastQC (version 0.11.5)