FastQCFastQC Report
Thu 2 Jun 2016
DRR028452_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028452_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1742283
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21450.073.6489941
GTATCAA38500.053.07941
TCAACGC40800.048.8032264
ATCAACG41400.048.270833
CAACGCA42550.046.966215
AACGCAG44300.044.293666
TATCAAC48600.041.5665442
ACGCAGA49850.038.7812777
CGCAGAG49600.038.100878
GCAGAGT61000.029.7933869
GTACATG40300.027.6919331
TACATGG43000.024.5844442
CAGAGTA59950.023.5516539
ACATGGG44350.022.5300163
GTATAGA11500.020.1646061
TTCGGCG2256.9305743E-419.308337
TAGTACG4954.820322E-1019.015784
GAGTACA33500.018.3870351
GTATATA19000.017.163131
GTATTAC12000.016.9088631
Produced by FastQC (version 0.11.5)