FastQCFastQC Report
Thu 2 Jun 2016
DRR028451_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028451_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1437577
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23150.1610348523939935No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15900.054.2360081
GTATCAA25200.051.476751
TCAACGC23150.048.4078754
ATCAACG23700.047.585343
CAACGCA24550.045.048345
AACGCAG25900.042.6895526
TATCAAC30800.038.7698752
TACATGG39450.036.2480662
GTACATG40150.036.1642651
ACGCAGA29050.036.0319567
CGCAGAG27800.034.7233778
ACATGGG44850.029.4185123
GCAGAGT34450.026.7574639
CATGGGA22650.024.7382414
CATGGGG27200.021.9553094
GTATATA15700.021.1257361
GTATAGA8950.020.5880851
AGTACCG1950.0062091218.90495
CAGAGTA30750.018.8831889
GTATAAG11100.018.592341
Produced by FastQC (version 0.11.5)