FastQCFastQC Report
Thu 2 Jun 2016
DRR028451_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028451_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1437577
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26080.1814163693492592No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24400.169730038808356No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22150.088.3034061
TAGCGCG300.00194641872.4011849
GTATCAA42450.059.898631
TCAACGC46600.054.067844
ATCAACG47550.053.139883
CAACGCA47600.052.931965
AACGCAG50950.049.3095446
TATCAAC55550.045.617312
CAACGCG657.387115E-444.554586
ACGCAGA57850.042.927587
CGCAGAG57250.042.2392968
GTACATG39550.035.5338821
GCAGAGT66450.035.192759
TACATGG41650.032.5066532
ACATGGG42900.030.2093523
CATGGGG25300.023.4659984
CATGGGA24900.021.5168194
GAGTACA31250.020.8631521
CAGAGTA63600.020.8324179
CGTATAC1800.004342115520.111443
Produced by FastQC (version 0.11.5)