FastQCFastQC Report
Thu 2 Jun 2016
DRR028450_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028450_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657990
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26700.1610383657320008No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16700.055.1709671
TCAACGC22700.048.3933144
ATCAACG23550.046.63363
CAACGCA23450.045.9037785
GTATCAA27550.042.539521
GTACATG42300.040.6006161
AACGCAG26100.040.386816
TACATGG43100.038.4776762
ACGCAGA28100.035.6771437
TATCAAC34650.034.672722
CGCAGAG28100.033.0499768
ACATGGG47250.031.8223273
CATGGGA23650.029.9271284
GCAGAGT35100.022.9468569
CATGGGG25650.022.132394
GTATATA18750.020.834911
GAGTACA30750.019.8952791
GTCTAAT12100.017.6656511
ATACAGT20750.016.6964366
GTATAGA12200.016.3125171
Produced by FastQC (version 0.11.5)