FastQCFastQC Report
Thu 2 Jun 2016
DRR028450_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028450_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657990
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21500.12967508851078716No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20180.12171364121617138No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21900.074.438811
GTATCAA43300.059.7367171
ATCAACG47850.053.2607573
TCAACGC48050.052.5870324
CAACGCA49600.050.9436875
AACGCAG52200.048.683666
TATCAAC58150.044.0757942
ACGCAGA58100.042.8675737
CGCAGAG57850.042.176758
GTACATG43600.034.3989261
GCAGAGT68300.033.7095579
TACATGG44100.033.4917762
ACATGGG44050.031.7218153
CATGGGA25600.024.3223254
CAGAGTA67700.023.8485959
CATGGGG24750.023.1099224
GAGTACA33200.020.2957881
TGGTATC11300.019.2215942
GTGGTAT12900.018.5346871
CCCGTCC2751.2558636E-418.4294328
Produced by FastQC (version 0.11.5)