FastQCFastQC Report
Thu 2 Jun 2016
DRR028449_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028449_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530937
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT9500.1789289501390937No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8820.1661214042344007No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6910.13014726794327763No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG5930.11168933413945534No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4150.094.1282351
GCTCTAG807.0904207E-964.4911651
GTATCAA10750.058.277731
TACATGA901.7960701E-857.331042
TACATGG21750.052.868822
GATGACC2100.051.8218469
GTACATG24100.050.7672041
ATCAACG11500.050.6364863
CATGGGA13150.049.3325234
TCAACGC11700.049.1456574
AACGCAT450.00902931149.1361277
CAACGCA11900.048.3196755
ACATGGA2200.046.9072153
TACTAGA3400.043.3554041
GGATGAC2600.041.852118
GTCACTC4700.041.677999
ACATGGG26050.041.5952643
TAGCGTG1053.8023518E-641.4574789
AGTCACT4750.041.2353448
AACGCAG13600.040.645696
Produced by FastQC (version 0.11.5)