FastQCFastQC Report
Thu 2 Jun 2016
DRR028449_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028449_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530937
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20260.3815895294545304No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19990.376504180345314No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT12190.22959409496795288No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10690.20134215547230652No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG9270.1745969860830946No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT7640.1438965451644922No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC7400.13937623484518877No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC7350.13843450352866724No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA7130.13429088573597242No Hit
TATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATATCATAG7020.13221907683962505No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG6310.11884649214501909No Hit
GTAAATATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATAT6160.11602129819545445No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA5810.10942917897980363No Hit
GACCATACCTATGTATCCAAATGGTTCTTTTTTTCCGGAGTAGTAAGTTA5530.10415548360728298No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10400.0127.477591
GTGGTAT1200.096.595051
GCTCTAG651.6370905E-1189.164661
TGGTATC1450.074.895032
GTATCAA27600.063.7842251
GTGTAAT350.003576065662.0968131
ACGCAGT350.003585421562.0558787
ATCGCAG350.003585421562.0558787
CACTTAC350.003585421562.0558787
ATCAACG32050.053.5365033
TCAACGC32550.052.714134
ACTTACC553.2399516E-452.6534738
CAACGCA32650.052.5526775
ATAGATG2950.051.537936
TACATGG21150.048.607992
GTACACT604.97723E-448.2656824
AACGCAG35550.048.0620276
TAGATGG3200.047.5115287
GATGACC2300.047.2164279
CTCTATA1551.4551915E-1146.708722
Produced by FastQC (version 0.11.5)