FastQCFastQC Report
Thu 2 Jun 2016
DRR028448_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028448_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1379892
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22280.16146191151191544No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13350.055.180031
TCAACGC18400.051.262664
ATCAACG19750.049.24193
GTATCAA21050.048.643621
CAACGCA19800.046.5232475
AACGCAG20450.045.3947076
TATCAAC26250.038.731252
ACGCAGA24000.037.453537
GTACATG34450.037.2068751
CGCAGAG23850.036.520848
TACATGG35800.033.749912
ACATGGG37900.031.1034643
GCAGAGT30800.026.1608629
GTATCGA2151.649668E-523.9840641
CATGGGA18000.023.3351884
TATACCG1901.9641491E-423.2714185
CATGGGG26450.022.2881134
GTATTAG10800.020.4625931
GAGTACA24350.020.2693121
ATAATAC12350.018.4936523
Produced by FastQC (version 0.11.5)