FastQCFastQC Report
Thu 2 Jun 2016
DRR028448_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028448_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1379892
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24350.17646308551683754No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21260.1540700286689103No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21050.083.289081
GTATCAA37450.054.166531
TCAACGC43750.045.510524
ATCAACG44050.045.364943
CAACGCA44300.045.1089325
AACGCAG46650.042.370956
TATCAAC50200.040.2399752
GTACATG34350.039.018431
ACGCAGA53650.036.7076347
CGCAGAG54550.035.305648
TACATGG37800.035.05232
ACATGGG38750.032.511333
GCAGAGT63200.029.2132789
CATGGGG25400.026.2247474
GTGGTAT9500.024.4034481
CAGAGTA60700.023.3789279
GAGTACA29400.020.9457741
CATGGGA20650.020.6866024
TGGTATC10550.020.588562
AGTACAT30450.018.5466082
Produced by FastQC (version 0.11.5)