FastQCFastQC Report
Thu 2 Jun 2016
DRR028447_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028447_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13433
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.5211047420531527No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA260.1935531899054567No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA230.17122012953175017No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA180.13399836224223927No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC170.12655400878433706No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN170.12655400878433706No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA160.1191096553264349No Hit
CAGTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTA140.10422094841063052No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAGCG100.0066124704147.864874
CATGGGA203.4964902E-4110.898654
GGTATCA258.491044E-488.718911
GTACATG400.005476585655.4493261
TACATGG450.00872590749.288292
AGAGTAC351.2347147E-424.86818310-14
CGACGAA2600.0078633488.275064145
CCGACGA2650.0095755845.9538827140-144
Produced by FastQC (version 0.11.5)