FastQCFastQC Report
Thu 2 Jun 2016
DRR028447_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028447_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13433
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1140.8486562942008486No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1040.7742127596218268No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT630.46899426784783743No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT580.43177250055832656No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.2754410779423807No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC310.2307749571949676No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA310.2307749571949676No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA280.20844189682126105No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA250.18610883644755452No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.17866448298965235No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT240.17866448298965235RNA PCR Primer, Index 17 (95% over 21bp)
CAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGT190.14144271570014144No Hit
GTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGG180.13399836224223927No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA170.12655400878433706No Hit
GCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCAC170.12655400878433706No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA160.1191096553264349No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.1191096553264349No Hit
ACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGGTGATGCGATGA140.10422094841063052No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT140.10422094841063052RNA PCR Primer, Index 17 (95% over 23bp)
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT140.10422094841063052No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAG140.10422094841063052No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC140.10422094841063052No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGAG100.007032456144.86942
CCAGAGG100.007032456144.86943
TGGAGTA100.007032456144.86942
AGTACAG100.007032456144.86945
CTGGAGT100.007032456144.86941
CGGGAGC100.007032456144.86943
TCTGAGC100.007032456144.86948
GGAGTAC100.007032456144.86943
GGTATCA700.0113.825951
GTATCAA1150.069.285372
TCAACGC1500.053.118785
TATCAAC1500.053.118783
ATCAACG1500.053.118784
AAGGCCG450.00946812448.2898035
AACGCAG1700.046.8695147
CAACGCA1700.046.8695146
ACGCAGA2003.6379788E-1239.839098
CGCAGAG2003.6379788E-1239.839099
GCAGAGT2201.9508661E-829.632389
AGAGTAC2100.018.68183310-14
Produced by FastQC (version 0.11.5)