FastQCFastQC Report
Thu 2 Jun 2016
DRR028446_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028446_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583063
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9560.16396169882156816No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6150.079.13071
GTATCAA16200.065.087441
CCGATCG350.003686380361.623108145
TCAACGC16800.058.812784
CAACGCA17150.057.1876265
ATCAACG17150.057.177523
GTACATG35300.050.5494961
TACATGG34200.050.4461022
AACGCAG19000.049.6743936
TATCAAC20850.049.506222
CATGGGA20600.043.6685524
CGCAGAG19850.043.1653378
ACGCAGA21400.041.6915257
ACATGGG40950.040.5103873
GCAGAGT20850.037.9639939
CATGGGG16650.035.4284324
GATGACC3300.035.2093059
AGTACAT23800.031.288322
TATATCG950.004348789331.0492365
ATATCGC1000.005591041429.494176
Produced by FastQC (version 0.11.5)