FastQCFastQC Report
Thu 2 Jun 2016
DRR028446_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028446_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583063
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25220.4325433100711244No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23830.40870369068179596No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12430.21318451007867073No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG6210.10650650101275505No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13350.0103.653551
GTATCAA32550.071.224771
TCAACGC40750.056.6854554
ATCAACG40900.056.654613
CAACGCA41650.055.460565
GAGTACG400.00606650654.336711
TATCAAC43300.053.3471642
TGGTATC3350.051.8770142
AACGCAG45150.051.6424336
GTGGTAT3400.051.1404381
GTACATG37000.046.4064871
TACATGG36450.046.287842
CGCAGAG50450.045.3559728
GTACGGA1602.0008883E-1145.2572946
ACGCAGA51250.045.071857
CATGGGA21100.042.5547264
ACATGGG38950.041.4577753
GCAGAGT53750.041.0894179
TCTGTCG2057.2759576E-1238.8550458
GTCTCGC1801.2988312E-732.1995351
Produced by FastQC (version 0.11.5)