FastQCFastQC Report
Thu 2 Jun 2016
DRR028445_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028445_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17018
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA580.3408156070043483No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.3231872135386062No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA540.3173110823833588No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA440.25854977083088493No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC400.2350452462098954No Hit
CAGTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTA310.18216006581266894No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN290.17040780350217416No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA270.1586555411916794No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA260.15277941003643203No Hit
GGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAAT230.13515101657068984No Hit
ATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATC230.13515101657068984No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA190.11164649194970032No Hit
CGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCCGC180.10577036079445293No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTGTA100.0066579483147.647427
ATGACAG100.0066579483147.647426
GAACCTG100.0066579483147.647425
CTGTAAC100.0070287487145.002999
TTCACAC100.0071553304144.14243145
GGTATCA255.736314E-6118.117931
GTATCAA451.0601059E-465.621082
ATCAACG501.7853612E-459.0589644
TCAACGC501.7853612E-459.0589645
ACGCAGA501.9532727E-458.0011988
AACGCAG552.8594286E-453.689977
TATCAAC701.4586789E-552.731223
CATGGGA450.00881912449.215811
CAACGCA656.5194775E-445.4299746
GTACATG709.391327E-442.184981
CCTTTGG800.001810794836.9118542
GCCTTTG950.0042054631.083671
CGCGATG200.00602492629.01792130-34
AGAGTAC500.001378817517.40035810-14
TGCCGAC3100.00575811713.949268145
Produced by FastQC (version 0.11.5)