FastQCFastQC Report
Thu 2 Jun 2016
DRR028444_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028444_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2208984
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35970.16283504090568332No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC25150.054.8050884
CAACGCA26900.051.24095
ATCAACG26900.050.9538273
AACGCAG28700.047.7603386
GTATCAA31500.047.2561
GGTATCA20400.042.9865841
CGCAGAG30950.040.560018
ACGCAGA32750.039.60487
GTACATG46750.038.3037071
TATCAAC39400.037.031822
TACATGG48500.033.122322
ACATGGG50050.030.624953
GCAGAGT40200.027.6177279
GTACTAG7750.021.869671
CATGGGA27000.021.5665454
GAGTACA35000.020.4230121
CGGTCGC2608.60087E-519.362041145
CATGGGG32700.018.9342784
GTATTAG18600.017.0361561
GTATTAA27800.016.4347461
Produced by FastQC (version 0.11.5)