FastQCFastQC Report
Thu 2 Jun 2016
DRR028444_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028444_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2208984
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24550.060.7964631
TCAACGC40800.056.2544484
ATCAACG42550.054.110973
GTATCAA43400.054.0901151
CAACGCA42600.053.8775025
AACGCAG46250.049.1559076
CGCAGAG51000.042.4481548
ACGCAGA52400.041.8667377
TATCAAC55500.041.745992
GTACATG44900.033.5644571
GCAGAGT63900.032.972359
TACATGG47800.029.5368732
ACATGGG46900.028.7142773
CATGGGG29400.021.1791674
CAGAGTA64650.019.1507329
TATACAG23950.019.0455175
GAGTACA34350.018.3508051
CTATACT19050.017.1031174
GTATTAG17800.017.0958961
AGAGTAC56700.016.02793310-14
Produced by FastQC (version 0.11.5)