FastQCFastQC Report
Thu 2 Jun 2016
DRR028443_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028443_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11690
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA870.7442258340461934No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA490.41916167664670656No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA460.39349871685201027No Hit
GTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGG230.19674935842600513No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA230.19674935842600513No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA210.17964071856287425No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC170.1454234388366125No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN170.1454234388366125No Hit
TGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCC170.1454234388366125No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA160.13686911890504705No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAG160.13686911890504705No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA140.11976047904191617No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC130.11120615911035071No Hit
GCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCAC130.11120615911035071No Hit
CAGTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTA130.11120615911035071No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA130.11120615911035071No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG120.1026518391787853No Hit
CGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCCGC120.1026518391787853No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC120.1026518391787853No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGACA100.0066958163147.163734
AACAAGG100.0066958163147.163736
TCAAGAC100.0066958163147.163733
CTCCTAG100.0066958163147.163736
GACATTC100.0066958163147.163737
CGTCGGA100.0066958163147.163732
CGCATAG100.006875346145.872829
GGTATCA203.5512645E-4110.372791
AGACATT258.6239487E-488.2982256
GTATCAA350.00327782363.0701682
AAGACAT350.00327782363.0701685
CGGTAAA350.00327782363.0701682
TCAACGC400.005562085755.1863945
ATCAACG400.005562085755.1863944
GTAAATG400.005562085755.1863944
GAGTACA450.008862019549.054571
TATCAAC450.008862019549.054573
GGGAGCT350.003493520421.0233882
CGCATTG551.16949406E-418.54935835-39
TCCGCGC551.2340434E-418.42020615-19
Produced by FastQC (version 0.11.5)