FastQCFastQC Report
Thu 2 Jun 2016
DRR028442_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028442_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537273
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8890.16546522903626276No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG6310.11744494884351159No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5710.10627744182194156No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4000.093.940921
TCGCTAG300.001999586471.90474145
GCTCTAG754.267349E-968.7672041
GATGACC1850.066.70359
ATCAACG10900.053.3953743
CAACGCA11200.053.316415
GTATAAA1257.6397555E-1153.048991
TCAACGC11000.052.945414
GTATCAA11700.052.897851
AACGCAG12450.046.7701266
GGATGAC2650.046.5665938
TACATGA951.7184448E-646.5297472
TACATGG20650.046.379532
CATGGGA12900.045.7187354
GTACATG24150.043.3227271
ATAGATG2950.042.475426
ACGCAGA12350.042.3742647
CGCAGAG11950.041.305938
GTACATA2003.6379788E-1240.5235331
AACGCAA959.318695E-538.800775
Produced by FastQC (version 0.11.5)