FastQCFastQC Report
Thu 2 Jun 2016
DRR028442_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028442_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537273
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18970.3530793469986394No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17080.3179016998806938No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG10600.19729262404773734No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10150.18891699378155985No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT7790.14499146616338435No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC7140.13289333355668348No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG6930.12898470609913396No Hit
ATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGG6490.1207952009499826No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT6310.11744494884351159No Hit
ACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGC5990.11148894509867423No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC5980.11130281998164807No Hit
CCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGC5950.11074444463056957No Hit
CTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCC5560.10348556506654903No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA5490.10218268924736586No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG5460.10162431389628736No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8450.0113.989071
CGCTATA259.4317476E-486.889772
GTGGTAT1650.074.616221
TGGTATC1700.072.408152
GCTCTAG1050.068.972981
TCATGGA851.70985E-1068.148842
TGGGAAC851.70985E-1068.148846
GTATCAA22550.062.94741
CATGGGA12200.056.383394
TACATGG19150.056.338452
ATGTCGG1101.6916601E-952.6604657
ATCAACG28100.049.7322853
ATAGATG2800.049.1340986
CAACGCA28500.049.0342865
GTACATG23500.049.0001641
TCAACGC28800.048.5235144
TATATCA450.00968511148.27212
ACATGGG21600.047.601653
GAAGTGT2450.047.2869536
TGTCGGG1255.2477844E-946.3412138
Produced by FastQC (version 0.11.5)