FastQCFastQC Report
Thu 2 Jun 2016
DRR028441_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028441_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1210180
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20170.1666694210778562No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12850.049.3720171
TCAACGC17650.048.0845154
ATCAACG17950.047.673723
CAACGCA18950.044.787755
AACGCAG19550.043.77956
GTATCAA20150.042.1025431
GTACATG32250.038.8870281
TACATGG32350.037.854662
ACGCAGA22050.036.811267
TATCAAC23150.036.6464922
ACATGGG33650.035.296073
CGCAGAG23050.033.71928
GCAGAGT26500.027.688199
CATGGGA18800.025.5157094
CATGGGG23300.025.3388084
TATACTG9850.020.9793685
GAGTACA22950.020.893741
GTATTAA12000.020.287021
GTCTAGA6900.019.2446021
AGTACAT24400.019.0474242
Produced by FastQC (version 0.11.5)