FastQCFastQC Report
Thu 2 Jun 2016
DRR028441_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028441_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1210180
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14740.12180006280057512No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13570.11213207952535988No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15800.079.328811
GTATCAA30050.062.686041
ATCAACG34950.052.6188433
TCAACGC35250.052.3764234
CAACGCA36850.049.9057925
AACGCAG38100.048.0784346
TATCAAC40400.045.5205082
ACGCAGA43150.041.9482577
CGCAGAG44300.040.5324368
GTACATG33650.038.1092261
TACATGG36600.034.2231372
GCAGAGT50400.033.471889
ACATGGG36150.032.8465923
ATACCGG900.003652472332.1789746
GTACGGT1000.00611494428.9610799
CATGGGG21900.024.1342324
TATACCG1801.5344593E-424.1342325
CAGAGTA49550.023.8176389
GTGGTAT8100.023.2557471
GAGTACA26050.022.5278241
Produced by FastQC (version 0.11.5)