FastQCFastQC Report
Thu 2 Jun 2016
DRR028440_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028440_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1863477
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30290.16254560694873077No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC25500.049.1183854
ATCAACG25700.048.7173233
GTATCAA28700.048.5019841
CAACGCA25650.048.257995
GGTATCA17200.047.5306931
AACGCAG27050.045.4754186
ACGCAGA30600.037.792517
TATCAAC34500.037.7841642
CGCAGAG30650.037.6425828
GTACATG45500.035.287811
TACATGG49050.031.8311632
ACATGGG51600.028.9743673
GCAGAGT39400.024.6793179
GAGTACA34950.024.2342871
CATGGGA23550.021.8999164
TCTAGCG1800.0039334520.4580923
CATGGGG35400.020.188494
AGTACAT41050.019.1966882
CAGAGTA38050.019.070849
ATAATAC20500.018.3224663
Produced by FastQC (version 0.11.5)