FastQCFastQC Report
Thu 2 Jun 2016
DRR028440_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028440_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1863477
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22650.077.4185941
GTATCAA43500.055.1362041
TCAACGC46150.050.9925464
ATCAACG47000.050.070343
CAACGCA47850.049.1808975
AACGCAG50450.046.5027736
TATCAAC55400.042.7398532
CGCAGAG57650.039.6901558
ACGCAGA58400.039.3044247
GTACATG45250.034.268361
GCAGAGT71300.030.6699079
TACATGG48600.030.0960962
ACATGGG49350.028.6116243
CATTGCG2101.5711423E-524.136479
CATGGGG33700.022.1310674
CAGAGTA68050.020.3235849
ATAATAC19050.019.76533
GAGTACA36750.019.5198271
AATGCGT3351.331513E-619.4532766
GTGGTAT14300.019.2550951
Produced by FastQC (version 0.11.5)