FastQCFastQC Report
Thu 2 Jun 2016
DRR028439_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028439_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1601142
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26080.16288374172934067No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC24300.058.2575234
CAACGCA24700.057.0155685
GTATCAA26000.056.989651
ATCAACG25100.056.097943
AACGCAG26650.050.8955656
GGTATCA14500.049.3147741
TATCAAC33000.044.2321932
GTACATG42900.042.2718351
ACGCAGA30700.041.2999427
CGCAGAG29600.039.7289058
TACATGG45750.037.5452
ACATGGG49600.031.6581213
CATGGGG30950.028.5875974
GCAGAGT36850.026.791599
GAGTACA29850.025.6839791
CATGGGA25000.022.7095094
GTATTAA18950.021.7847271
GTATAGG8800.020.1048981
AGTACAT34900.019.8559062
GTATATA16350.018.4858471
Produced by FastQC (version 0.11.5)