FastQCFastQC Report
Thu 2 Jun 2016
DRR028439_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028439_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1601142
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24360.1521414090692768No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21500.13427915825079847No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23750.083.6097261
GTATCAA45350.060.2467881
ATCAACG49400.055.1125953
TCAACGC49150.054.9509544
CAACGCA50600.053.6624765
AACGCAG53350.050.4891976
TATCAAC59100.046.189562
ACGCAGA61350.043.6693887
CGCAGAG61450.043.009158
GTACATG43750.037.933881
GCAGAGT69500.036.4647459
TACATGG46200.035.420642
ACATGGG45900.033.4436073
CATGGGG30250.024.6548164
CATGGGA23850.024.5915874
GTACGAC1500.001505689624.1361873
GTGGTAT11850.023.851521
GAGTACA31600.021.3287661
CTATACG1800.00433976820.113494
CAGAGTA69450.019.8093999
Produced by FastQC (version 0.11.5)