FastQCFastQC Report
Thu 2 Jun 2016
DRR028438_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028438_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13226
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.6199909269620445No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA520.39316497807349154No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA430.32511719340692574No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA340.2570694087403599No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA250.18902162407379403No Hit
GGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAAT210.15877816422198698No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC170.12853470437017994No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN170.12853470437017994No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA150.11341297444427642No Hit
CAGTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTA150.11341297444427642No Hit
TGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCC150.11341297444427642No Hit
ATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATC140.10585210948132466No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATTCC100.006776074146.658924
CAGAGAC100.006776074146.658923
CCTCCAT100.006776074146.658921
TCCATGC100.006776074146.658923
TACCCTC100.006776074146.658927
AGGTAAT100.006776074146.658927
TAGATTA100.006776074146.658926
GCAGAGT406.303945E-572.765388
ACGCAGA451.0869547E-465.181746
TCAACGC451.0869547E-465.181743
CATGGGA451.0869547E-465.181744
TCCGCGC350.003437727262.370338
CGCAGAG501.8305526E-458.663577
CCGCGCG400.005833002754.5740369
ATCAACG552.9317656E-453.3305132
CAGAGTA553.0466207E-452.920289
GTATCAA450.00901213448.8863071
AACGCAG803.3028526E-545.830915
TATCAAC803.3028526E-545.830911
GTACATG656.684222E-445.125821